Real-Time Quantitative PCR and Fluorescence In Situ Hybridization for Subcellular Localization of miRNAs in Neurons.

Autor:	Tatarakis A; Department of Cell Biology, and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA. antonis_tatarakis@hms.harvard.edu., Moazed D; Department of Cell Biology, and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA. danesh@hms.harvard.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2417, pp. 1-17.
DOI:	10.1007/978-1-0716-1916-2_1
Abstrakt:	Neuronal miRNAs play major roles in regulation of synaptic development and plasticity. The small size of miRNAs and, in some cases, their low level of expression make their quantification and detection challenging. Here, we outline methods to quantify steady state levels of miRNAs in neurons and the brain by using real-time quantitative PCR (RT-qPCR) and to determine miRNA subcellular localization in primary neurons by a sensitive fluorescence in situ hybridization (FISH) method. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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