An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.

Autor:	Majbour D; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Suarez-Martinez AD; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Hodges NA; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Lampejo AO; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Lomel BM; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Rice EW; Department of Plastic and Reconstructive Surgery, Wake Forest School of Medicine, Winston-Salem, NC, USA., Shang H; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Katz AJ; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA., Murfee WL; Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA. wmurfee@bme.ufl.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2441, pp. 157-170.
DOI:	10.1007/978-1-0716-2059-5_12
Abstrakt:	Stromal vascular fraction (SVF), isolated from adipose tissue, identifies as a rich cell source comprised of endothelial cells, endothelial progenitor cells, pericytes, smooth muscle cells, fibroblasts, and immune cells. SVF represents a promising therapeutic heterogonous cell source for growing new blood microvessels due to its rich niche of cells. However, the spatiotemporal dynamics of SVF within living tissues remain largely unknown. The objective of this chapter is to describe a protocol for culturing SVF on mouse mesentery tissues in order to aid in the discovery of SVF dynamics and associated vessel growth over time. SVF was isolated from the inguinal adipose from adult mice and seeded onto mesentery tissues. Tissues were then cultured for up to 5 days and labeled with endothelial cell and pericyte markers. Representative results demonstrate the observation of SVF-derived vasculogenesis characterized by de novo vessel formation and subsequent vessel connection. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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