Staphylococcus aureus and Acinetobacter baumannii Inhibit Osseointegration of Orthopedic Implants.

Autor:	Choe H; Department of Orthopaedics, Case Western Reserve Universitygrid.67105.35, Cleveland, Ohio, USA.; Department of Orthopaedics, Yokohama City University, Yokohama, Kanagawa, Japan., Tatro JM; Department of Orthopaedics, Case Western Reserve Universitygrid.67105.35, Cleveland, Ohio, USA., Hausman BS; Department of Orthopaedics, Case Western Reserve Universitygrid.67105.35, Cleveland, Ohio, USA., Hujer KM; CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA., Marshall SH; CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA., Akkus O; Department of Mechanical Engineering, Case Western Reserve University, Cleveland, Ohio, USA., Rather PN; Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia, USA.; Research Service, Atlanta Veterans Affairs Medical Center, Decatur, Georgia, USA., Lee Z; Department of Radiology, Case Western Reserve University, Cleveland, Ohio, USA., Bonomo RA; CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA.; Medical Service and GRECC, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.; Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.; Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.; Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.; Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.; Center for Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA., Greenfield EM; Department of Orthopaedics, Case Western Reserve Universitygrid.67105.35, Cleveland, Ohio, USA.; Department of Orthopaedic Surgery, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2022 Mar 17; Vol. 90 (3), pp. e0066921. Date of Electronic Publication: 2022 Jan 31.
DOI:	10.1128/iai.00669-21
Abstrakt:	Bacterial infections routinely cause inflammation and thereby impair osseointegration of orthopedic implants. Acinetobacter spp., which cause osteomyelitis following trauma, on or off the battlefield, were, however, reported to cause neither osteomyelitis nor osteolysis in rodents. We therefore compared the effects of Acinetobacter strain M2 to those of Staphylococcus aureus in a murine implant infection model. Sterile implants and implants with adherent bacteria were inserted in the femur of mice. Bacterial burden, levels of proinflammatory cytokines, and osseointegration were measured. All infections were localized to the implant site. Infection with either S. aureus or Acinetobacter strain M2 increased the levels of proinflammatory cytokines and the chemokine CCL2 in the surrounding femurs, inhibited bone formation around the implant, and caused loss of the surrounding cortical bone, leading to decreases in both histomorphometric and biomechanical measures of osseointegration. Genetic deletion of TLR2 and TLR4 from the mice partially reduced the effects of Acinetobacter strain M2 on osseointegration but did not alter the effects of S. aureus. This is the first report that Acinetobacter spp. impair osseointegration of orthopedic implants in mice, and the murine model developed for this study will be useful for future efforts to clarify the mechanism of implant failure due to Acinetobacter spp. and to assess novel diagnostic tools or therapeutic agents.
Databáze:	MEDLINE
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