Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent.

Autor: Shajib MS; School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia.; Centre for Biomedical Technologies, School of Mechanical, Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, Australia.; Translational Research Institute, Brisbane, QLD, Australia., Futrega K; Centre for Biomedical Technologies, School of Mechanical, Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, Australia.; Translational Research Institute, Brisbane, QLD, Australia.; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Jacob Klein T; Centre for Biomedical Technologies, School of Mechanical, Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, Australia., Crawford RW; Centre for Biomedical Technologies, School of Mechanical, Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, Australia., Doran MR; School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, QLD, Australia.; Centre for Biomedical Technologies, School of Mechanical, Medical, and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, Australia.; Translational Research Institute, Brisbane, QLD, Australia.; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.; Mater Research Institute - University of Queensland, Brisbane, QLD, Australia.
Jazyk: angličtina
Zdroj: Journal of tissue engineering [J Tissue Eng] 2022 Jan 23; Vol. 13, pp. 20417314221074207. Date of Electronic Publication: 2022 Jan 23 (Print Publication: 2022).
DOI: 10.1177/20417314221074207
Abstrakt: When repairing cartilage defects a major challenge is achieving high-quality integration between the repair tissue and adjacent native cartilage. Matrix-rich cartilage is not easily remodeled, motivating several studies to trial enzyme treatment of the tissue interface to facilitate remodeling and integration. Studying and optimizing such processes is tedious, as well as potentially expensive, and thus simpler models are needed to evaluate the merits of enzyme treatment on cartilage tissue integration. Herein, we used engineered cartilage microtissues formed from bone marrow-derived stromal cells (BMSC) or expanded articular chondrocytes (ACh) to study the impact of enzyme treatment on cartilage tissue integration and matrix remodeling. A 5-min treatment with collagenase appeared to improve cartilage microtissue integration, while up to 48 h treatment with hyaluronidase did not. Alcian blue and anti-collagen II staining suggested that collagenase treatment did facilitate near seamless integration of cartilage microtissues. Microtissue sections were stained with Picrosirius red and characterized using polarized light microscopy, revealing that individual microtissues contained a collagen network organized in concentric shells. While collagenase treatment appeared to improve tissue integration, assessment of the collagen fibers with polarized light indicated that enzymatically damaged networks were not remodeled nor restored during subsequent culture. This model and these data paradoxically suggest that collagen network disruption is required to improve cartilage tissue integration, but that the disrupted collagen networks are unlikely to subsequently be restored. Future studies should attempt to limit collagen network disruption to the surface of the cartilage, and we recommend using Picrosirius red staining and polarized light to assess the quality of matrix remodeling and integration.
Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(© The Author(s) 2022.)
Databáze: MEDLINE
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