Physiological Aspects of Germination and Early Seedling Establishment of Pleurotus sajor-caju Glyceraldehyde-3-Phosphate Dehydrogenase Expressing Transgenic Rice in Saline Environment.

Autor:	Siddiqui ZS; Stress Physiology and Phenomic Laboratory, Department of Botany, University of Karachi, Karachi, Pakistan., Lee GS; Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, South Korea., Cho W; Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, South Korea., Jeong MJ; Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, South Korea., Park SC; Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, South Korea., Kwon TR; Technology Cooperation Bureau, Rural Development Administration, Jeonju, South Korea., Zulfiqar F; Department of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, Pakistan., Umar M; Stress Physiology and Phenomic Laboratory, Department of Botany, University of Karachi, Karachi, Pakistan., Abideen Z; Dr. Muhammad Ajmal Khan Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi, Pakistan., Uddin Z; Department of Physics, University of Karachi, Karachi, Pakistan., Ansari HH; Stress Physiology and Phenomic Laboratory, Department of Botany, University of Karachi, Karachi, Pakistan., Wajid D; Stress Physiology and Phenomic Laboratory, Department of Botany, University of Karachi, Karachi, Pakistan., Cho JI; Crop Production and Physiology Division, National Institute of Crop Science, Rural Development Administration, Wanju, South Korea.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Jan 12; Vol. 12, pp. 767826. Date of Electronic Publication: 2022 Jan 12 (Print Publication: 2021).
DOI:	10.3389/fpls.2021.767826
Abstrakt:	GPD encodes glyceraldehyde-3-phosphate dehydrogenase enzyme involved in sugar mobilization, particularly glycolysis and gluconeogenesis. The objective of this study was to determine physiological aspects of germination and early seedling establishment of PsGPD ( Pleurotus sajor-caju glyceraldehyde-3-phosphate dehydrogenase) expressing transgenic rice (T5) against different salt concentrations. The T5 line that carried 2 copies of T-DNA and had the highest level of PsGPD expression was used in the investigation. Final germination percentage, amylase activity, reducing sugar accumulation, and chlorophyll biosynthesis were comparatively higher in PsGPD expressing transgenic rice against elevating saline conditions. A slow-paced conversion of porphyrin's precursors was seen through the matrix model and further elaborated by a graphical model. A sustained level of porphyrin was observed in PsGPD expressing transgenic rice. These data were concurrent with the relative gene expression and thermal imaging (thermography) of PsGPD expressing transgenic rice against salt stress. Morphological attributes also favored the salt tolerance exhibited by PsGPD- transformed rice. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Siddiqui, Lee, Cho, Jeong, Park, Kwon, Zulfiqar, Umar, Abideen, Uddin, Ansari, Wajid and Cho.)
Databáze:	MEDLINE
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