Self-Induced Back-Action Actuated Nanopore Electrophoresis (SANE) Sensor for Label-Free Detection of Cancer Immunotherapy-Relevant Antibody-Ligand Interactions.

Autor:	Peri SSS; Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX, USA., Raza MU; Department of Electrical Engineering, University of Texas at Arlington, Arlington, TX, USA., Sabnani MK; Department of Biology, University of Texas at Arlington, Arlington, TX, USA., Ghaffari S; Department of Biology, University of Texas at Arlington, Arlington, TX, USA., Gimlin S; Resonant Sensors Incorporated (RSI), Arlington, TX, USA., Wawro DD; Resonant Sensors Incorporated (RSI), Arlington, TX, USA., Lee JS; Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA., Kim MJ; Department of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA., Weidanz J; Department of Kinesiology, University of Texas at Arlington, Arlington, TX, USA., Alexandrakis G; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USA. galex@uta.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2394, pp. 343-376.
DOI:	10.1007/978-1-0716-1811-0_20
Abstrakt:	We fabricated a novel single molecule nanosensor by integrating a solid-state nanopore and a double nanohole nanoaperture. The nanosensor employs Self-Induced Back-Action (SIBA) for optical trapping and enables SIBA-Actuated Nanopore Electrophoresis (SANE) for concurrent acquisition of bimodal optical and electrical signatures of molecular interactions. This work describes how to fabricate and use the SANE sensor to quantify antibody-ligand interactions. We describe how to analyze the bimodal optical-electrical data to improve upon the discrimination of antibody and ligand versus bound complex compared to electrical measurements alone. Example results for specific interaction detection are described for T-cell receptor-like antibodies (TCRmAbs) engineered to target peptide-presenting Major Histocompatibility Complex (pMHC) ligands, representing a model of target ligands presented on the surface of cancer cells. We also describe how to analyze the bimodal optical-electrical data to discriminate between specific and non-specific interactions between antibodies and ligands. Example results for non-specific interactions are shown for cancer-irrelevant TCRmAbs targeting the same pMHCs, as a control. These example results demonstrate the utility of the SANE sensor as a potential screening tool for ligand targets in cancer immunotherapy, though we believe that its potential uses are much broader. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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