Systemic effect of TiO 2 micro- and nanoparticles after acute exposure in a murine model.

Autor: Domingo MG; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Anatomía Patológica, Buenos Aires, Argentina.; Becario de Investigación de la Universidad de Buenos Aires, Buenos Aires, Argentina., Kurtz M; CONICET, Buenos Aires, Argentina.; Universidad Nacional de San Martín, Escuela de Ciencia y Tecnología, Buenos Aires, Argentina.; CONICET - Universidad Nacional de San Martín, Instituto de Tecnologías Emergentes y Ciencias Aplicadas (ITECA), San Martín, Buenos Aires, Argentina., Maglione G; Universidad Nacional de San Martín, Escuela de Ciencia y Tecnología, Buenos Aires, Argentina.; CONICET - Universidad Nacional de San Martín, Instituto de Tecnologías Emergentes y Ciencias Aplicadas (ITECA), San Martín, Buenos Aires, Argentina.; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Histología y Embriología, Buenos Aires, Argentina., Martin M; CONICET, Buenos Aires, Argentina., Brites F; CONICET, Buenos Aires, Argentina.; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica, Laboratorio de Lípidos y Lipoproteínas, Buenos Aires, Argentina., Tasat DR; Universidad Nacional de San Martín, Escuela de Ciencia y Tecnología, Buenos Aires, Argentina.; CONICET - Universidad Nacional de San Martín, Instituto de Tecnologías Emergentes y Ciencias Aplicadas (ITECA), San Martín, Buenos Aires, Argentina.; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Histología y Embriología, Buenos Aires, Argentina., Olmedo DG; Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Anatomía Patológica, Buenos Aires, Argentina.; CONICET, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2022 Jul; Vol. 110 (7), pp. 1563-1572. Date of Electronic Publication: 2022 Jan 28.
DOI: 10.1002/jbm.b.35017
Abstrakt: The surface of a biomedical implant can be a potential endogenous source of release of microparticles (MPs) and nanoparticles (NPs) into the biological environment. In addition, titanium particles from exogenous sources can enter the body through inhalation, ingestion, or dermal contact. The aim of this work was to evaluate the biological response of the lung, liver, and kidneys to acute exposure to titanium dioxide (TiO 2 ). Male Wistar rats were intraperitoneally injected with a suspension of 45 μm or 5 nm TiO 2 particles. One month post-exposure, titanium concentration was determined spectrometrically (ICP-MS) in plasma and target organs. Blood smears and organ tissue samples were examined histopathologically, and oxidative metabolism was analyzed (superoxide anion by nitro blue tetrazolium (NBT) test; superoxide dismutase (SOD) and catalase (CAT); lipid peroxidation; paraoxonase 1). Liver (aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase) and kidney (urea, creatinine) function was evaluated using serum biochemical markers. Microchemical and histological analysis revealed the presence of particles, though no structural alterations, in TiO 2 -exposed groups. NBT test showed an increase in the percentage of reactive cells and antioxidant enzyme consumption in lung samples in the 45 μm and 5 nm TiO 2 -exposed groups. Only the 5 nm particles caused a decrease in SOD and CAT activity in the liver. No changes in renal oxidative metabolism were observed in either of the TiO 2 -exposed groups. Determination of serum biochemical markers and analysis of oxidative metabolism are not early bioindicators of tissue damage caused by TiO 2 MPs and NPs.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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