Embryonic and early postnatal cranial bone volume and tissue mineral density values for C57BL/6J laboratory mice.

Autor: Lesciotto KM; College of Osteopathic Medicine, Sam Houston State University, Conroe, Texas, USA., Tomlinson L; Department of Biology, New York University, New York, New York, USA., Leonard S; College of Medicine, Drexel University, Philadelphia, Pennsylvania, USA., Richtsmeier JT; Department of Anthropology, Pennsylvania State University, University Park, Pennsylvania, USA.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2022 Jul; Vol. 251 (7), pp. 1196-1208. Date of Electronic Publication: 2022 Feb 07.
DOI: 10.1002/dvdy.458
Abstrakt: Background: Laboratory mice are routinely used in craniofacial research based on the relatively close genetic relationship and conservation of developmental pathways between humans and mice. Since genetic perturbations and disease states may have localized effects, data from individual cranial bones are valuable for the interpretation of experimental assays. We employ high-resolution microcomputed tomography to characterize cranial bones of C57BL/6J mice at embryonic day (E) 15.5 and E17.5, day of birth (P0), and postnatal day 7 (P7) and provide estimates of individual bone volume and tissue mineral density (TMD).
Results: Average volume and TMD values are reported for individual bones. Significant differences in volume and TMD during embryonic ages likely reflect early mineralization of cranial neural crest-derived and intramembranously forming bones. Although bones of the face and vault had higher TMD values during embryonic ages, bones of the braincase floor had significantly higher TMD values by P7.
Conclusions: These ontogenetic data on cranial bone volume and TMD serve as a reference standard for future studies using mice bred on a C57BL/6J genetic background. Our findings also highlight the importance of differentiating "control" data from mice that are presented as "unaffected" littermates, particularly when carrying a single copy of a cre-recombinase gene.
(© 2022 The Authors. Developmental Dynamics published by Wiley Periodicals LLC on behalf of American Association for Anatomy.)
Databáze: MEDLINE
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