Epigenomic and structural events preclude recombination in Brassica napus.

Autor: Boideau F; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Richard G; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Coriton O; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Huteau V; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Belser C; Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Université Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, Evry, 91057, France., Deniot G; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Eber F; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Falentin C; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Ferreira de Carvalho J; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Gilet M; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Lodé-Taburel M; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Maillet L; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Morice J; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Trotoux G; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Aury JM; Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Université Evry, Université Paris-Saclay, 2 Rue Gaston Crémieux, Evry, 91057, France., Chèvre AM; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France., Rousseau-Gueutin M; IGEPP, INRAE, Institut Agro, Univ Rennes, Le Rheu, 35653, France.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2022 Apr; Vol. 234 (2), pp. 545-559. Date of Electronic Publication: 2022 Feb 21.
DOI: 10.1111/nph.18004
Abstrakt: Meiotic recombination is a major evolutionary process generating genetic diversity at each generation in sexual organisms. However, this process is highly regulated, with the majority of crossovers lying in the distal chromosomal regions that harbor low DNA methylation levels. Even in these regions, some islands without recombination remain, for which we investigated the underlying causes. Genetic maps were established in two Brassica napus hybrids to detect the presence of such large nonrecombinant islands. The role played by DNA methylation and structural variations in this local absence of recombination was determined by performing bisulfite sequencing and whole genome comparisons. Inferred structural variations were validated using either optical mapping or oligo fluorescence in situ hybridization. Hypermethylated or inverted regions between Brassica genomes were associated with the absence of recombination. Pairwise comparisons of nine B. napus genome assemblies revealed that such inversions occur frequently and may contain key agronomic genes such as resistance to biotic stresses. We conclude that such islands without recombination can have different origins, such as DNA methylation or structural variations in B. napus. It is thus essential to take into account these features in breeding programs as they may hamper the efficient combination of favorable alleles in elite varieties.
(© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
Databáze: MEDLINE
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