Recombinant Expression of Complex Proteins in Human Cell Lines.

Autor:	de Sousa Bomfim A; School of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil.; Regional Blood Center of Ribeirão Preto, São Paulo, Brazil., Archangelo BS; School of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil., Pereira AS; School of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil., de Sousa Russo EM; School of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil. elisa@fcfrp.usp.br.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2406, pp. 327-336.
DOI:	10.1007/978-1-0716-1859-2_19
Abstrakt:	Coagulation factors, as factor VII, VIII, and IX, are complex proteins which are very difficult to express. Blood coagulation factor IX is a vitamin K-dependent protein, and it has become a valuable biopharmaceutical in the treatment of hemophilia B. Here, we describe the techniques used to generate human cell lines producing human recombinant factor IX, as an example of complex protein, as well as in vitro characterization of this coagulation factor.To produce the FIX human adherent 293T SK-Hep-1 cells were used and stably modified by a lentiviral vector carrying the hFIX and the eGFP genes. The eGFP was employed as a reporter protein. (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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