Herbicide tolerance and gene silencing stability over generations in the ricin bio-detoxicated castor bean.

Autor: de Sousa NL; Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, Brasília, DF, 70770-900, Brazil., Cabral GB; Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, Brasília, DF, 70770-900, Brazil., Aragão FJL; Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, Brasília, DF, 70770-900, Brazil. francisco.aragao@embrapa.br.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2022 Jan 28; Vol. 20 (1), pp. 16. Date of Electronic Publication: 2022 Jan 28.
DOI: 10.1186/s43141-022-00303-w
Abstrakt: Castor bean (Ricinus communis L.) is an important cultivated oilseed. Seeds contain ricinoleic acid, a valuable product for a variety of industries. Castor cake is a residue of ricinoleic manufacture and could be used as animal feed due to its high amount of protein. However, castor cake contains ricin and RCA 120 , both highly toxic and allergenic proteins. In 2017, we reported the development of a transgenic event (named TB14S-5D) with an undetectable amount of ricin/RCA 120. In the present work, we evaluate TB14S-5D for tolerance to the herbicide imazapyr, as it contains the selectable marker gene, ahas, which was previously isolated from Arabidopsis thaliana and contains a mutation at position 653 bp. In addition, we demonstrated that the ricin coding genes are stably silenced over three generations.
(© 2022. The Author(s).)
Databáze: MEDLINE
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