Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging.
| Autor: | Cole JD; Brain Research Institute, University of Zurich, Zurich, Switzerland., Sarabia Del Castillo J; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Gut G; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Gonzalez-Bohorquez D; Brain Research Institute, University of Zurich, Zurich, Switzerland., Pelkmans L; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Jessberger S; Brain Research Institute, University of Zurich, Zurich, Switzerland. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2022 Jan 28; Vol. 11. Date of Electronic Publication: 2022 Jan 28. |
| DOI: | 10.7554/eLife.68000 |
| Abstrakt: | Advancing age causes reduced hippocampal neurogenesis, associated with age-related cognitive decline. The spatial relationship of age-induced alterations in neural stem cells (NSCs) and surrounding cells within the hippocampal niche remains poorly understood due to limitations of antibody-based cellular phenotyping. We established iterative indirect immunofluorescence imaging (4i) in tissue sections, allowing for simultaneous detection of 18 proteins to characterize NSCs and surrounding cells in 2-, 6-, and 12-month-old mice. We show that reorganization of the dentate gyrus (DG) niche already occurs in middle-aged mice, paralleling the decline in neurogenesis. 4i-based tissue analysis of the DG identifies changes in cell-type contributions to the blood-brain barrier and microenvironments surrounding NSCs to play a pivotal role to preserve neurogenic permissiveness. The data provided represent a resource to characterize the principles causing alterations of stem cell-associated plasticity within the aging DG and provide a blueprint to analyze somatic stem cell niches across lifespan in complex tissues. Competing Interests: JC, JS, DG, SJ No competing interests declared, GG, LP Inventor of a patent related to 4i technology (WO2019207004A1) (© 2022, Cole et al.) |
| Databáze: | MEDLINE |
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