Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management.

Autor: Reavey CE; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Walker AS; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Joyce SP; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Broom L; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK.; Centre for Medicines Discovery, University of Oxford, Old Road Campus Research Build, Roosevelt Dr, Headington, Oxford, OX3 7DQ, UK., Willse A; Bayer Crop Science, Chesterfield, MO, 63017, USA., Ercit K; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Poletto M; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Barnes ZH; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Marubbi T; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Troczka BJ; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Treanor D; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Beadle K; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Granville B; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., de Mello V; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Teal J; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Sulston E; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Ashton A; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Akilan L; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Naish N; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Stevens O; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Humphreys-Jones N; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Warner SAJ; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK.; Oxford University Innovation, Buxton Court, 3 West Way, Oxford, OX2 0JB, UK., Spinner SAM; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Rose NR; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK. nathan.rose@oxitec.com., Head G; Bayer Crop Science, Chesterfield, MO, 63017, USA., Morrison NI; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK., Matzen KJ; Oxitec Ltd, 71 Innovation Drive, Milton Park, Abingdon, OX14 4RQ, UK. Kelly.matzen@oxitec.com.
Jazyk: angličtina
Zdroj: BMC biotechnology [BMC Biotechnol] 2022 Jan 27; Vol. 22 (1), pp. 5. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1186/s12896-022-00735-9
Abstrakt: Background: The fall armyworm, Spodoptera frugiperda, is a significant and widespread pest of maize, sorghum, rice, and other economically important crops. Successful management of this caterpillar pest has historically relied upon application of synthetic insecticides and through cultivation of genetically engineered crops expressing insecticidal proteins (Bt crops). Fall armyworm has, however, developed resistance to both synthetic insecticides and Bt crops, which risks undermining the benefits delivered by these important crop protection tools. Previous modelling and empirical studies have demonstrated that releases of insecticide- or Bt-susceptible insects genetically modified to express conditional female mortality can both dilute insecticide resistance and suppress pest populations.
Results: Here, we describe the first germline transformation of the fall armyworm and the development of a genetically engineered male-selecting self-limiting strain, OX5382G, which exhibits complete female mortality in the absence of an additive in the larval diet. Laboratory experiments showed that males of this strain are competitive against wild-type males for copulations with wild-type females, and that the OX5382G self-limiting transgene declines rapidly to extinction in closed populations following the cessation of OX5382G male releases. Population models simulating the release of OX5382G males in tandem with Bt crops and non-Bt 'refuge' crops show that OX5382G releases can suppress fall armyworm populations and delay the spread of resistance to insecticidal proteins.
Conclusions: This article describes the development of self-limiting fall armyworm designed to control this pest by suppressing pest populations, and population models that demonstrate its potential as a highly effective method of managing resistance to Bt crops in pest fall armyworm populations. Our results provide early promise for a potentially valuable future addition to integrated pest management strategies for fall armyworm and other pests for which resistance to existing crop protection measures results in damage to crops and impedes sustainable agriculture.
(© 2022. The Author(s).)
Databáze: MEDLINE
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