| Autor: |
Junior GLV; Laboratory of Enzymology and Fermentation Technology; Health Department, State University of Feira de Santana (UEFS), Bahia, Brazil., Santos JDG; Health Department, State University of Feira de Santana (UEFS), Bahia, Brazil.; University Veterinary Hospital, Federal University of Agreste of Pernambuco (UFAPE), Garanhuns, PE, Brazil., Chaves PFP; Biochemistry and Molecular Biology Department, Federal University of Paraná, Curitiba, PR, Brazil., Cordeiro LMC; Biochemistry and Molecular Biology Department, Federal University of Paraná, Curitiba, PR, Brazil., Boffo EF; Department of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, 40170-115, Salvador, BA, Brazil., de Assis SA; Laboratory of Enzymology and Fermentation Technology; Health Department, State University of Feira de Santana (UEFS), Bahia, Brazil. |
| Abstrakt: |
Mannans has been attracted the interest in various sectors due to its promising applications. The low toxicity of mannans allows for their use in cosmetics, pharmaceutical, and biomedical industries. In this study, the α-D-mannan extraction conditions from Aureobasidium pullulans by alkaline extraction were optimized using a Box-Behnken design (BBD). The effect of temperature (°C), pH and extraction time (hours) on the yield of α-mannan was investigated. The conditions that produced the highest yield (26%) were a temperature of 92 °C, extraction time of 3 h and pH 13. In addition, the α-D-mannan structure was confirmed by methylation analysis, 1D and 2D NMR spectroscopic analysis, and GC-MS. |
