Non-invasive breath collection in murine models using a newly developed sampling device .

Autor:	Hintzen KFH; Department of Pharmacology and Toxicology, Maastricht University Medical Centre, Maastricht, The Netherlands.; Department of Surgery, Maastricht University Medical Centre, PO Box 5800, 6202 AZ Maastricht, The Netherlands.; NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands., Smolinska A; Department of Pharmacology and Toxicology, Maastricht University Medical Centre, Maastricht, The Netherlands.; NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands., Mommers AGR; Department of Pharmacology and Toxicology, Maastricht University Medical Centre, Maastricht, The Netherlands.; NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands., Bouvy ND; Department of Surgery, Maastricht University Medical Centre, PO Box 5800, 6202 AZ Maastricht, The Netherlands.; GROW School for Oncology and Developmental Biology, Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands., van Schooten FJ; Department of Pharmacology and Toxicology, Maastricht University Medical Centre, Maastricht, The Netherlands.; NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands., Lubbers T; Department of Surgery, Maastricht University Medical Centre, PO Box 5800, 6202 AZ Maastricht, The Netherlands.; GROW School for Oncology and Developmental Biology, Maastricht University, PO Box 5800, 6202 AZ Maastricht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Journal of breath research [J Breath Res] 2022 Feb 14; Vol. 16 (2). Date of Electronic Publication: 2022 Feb 14.
DOI:	10.1088/1752-7163/ac4fae
Abstrakt:	Volatile organic compounds (VOCs) in exhaled breath have the potential to be used as biomarkers for screening and diagnosis of diseases. Clinical studies are often complicated by both modifiable and non-modifiable factors influencing the composition of VOCs in exhaled breath. Small laboratory animal studies contribute in obtaining fundamental insight in alterations in VOC composition in exhaled breath and thereby facilitate the design and analysis of clinical research. However, long term animal experiments are often limited by invasive breath collection methods and terminal experiments. To overcome this problem, a novel device was developed for non-invasive breath collection in mice using glass nose-only restrainers thereby omitting the need of anesthetics. C57Bl/6 J mice were used to test reproducibility and different air sampling settings for air-flow (ml min -1 ) and time (minutes). Exhaled air was collected on desorption tubes and analysed for VOCs by gas chromatography time-of-flight mass spectrometry (GC-tof-MS). In total 27 compounds were putatively identified and used to assess the variability of the VOC measurements in the breath collections. Best reproducibility is obtained when using an air flow of 185 ml min -1 and a collection time of 20 min. Due to the non-invasive nature of breath collections in murine models, this device has the potential to facilitate VOC research in relation to disturbed metabolism and or disease pathways. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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