Spatial and temporal immunoreaction of nestin, CD44, collagen IX and GFAP in human retinal Müller cells in the developing fetal eye.

Autor: Bulirsch LM; Department of Ophthalmology, University Hospital Bonn, Germany; Division of Ophthalmic Pathology, Department of Ophthalmology, University Hospital Bonn, Germany. Electronic address: louisa.bulirsch@ukbonn.de., Loeffler KU; Department of Ophthalmology, University Hospital Bonn, Germany; Division of Ophthalmic Pathology, Department of Ophthalmology, University Hospital Bonn, Germany., Holz FG; Department of Ophthalmology, University Hospital Bonn, Germany., Koinzer S; Augenarzt am Dreiecksplatz and Department of Ophthalmology, University Hospital of Schleswig-Holstein, Campus Kiel, Germany., Nadal J; Department of Medical Biometry, Informatics and Epidemiology, University Hospital Bonn, Germany., Müller AM; Center of Pediatric Pathology and Pathology, University Hospital Cologne, Germany., Herwig-Carl MC; Department of Ophthalmology, University Hospital Bonn, Germany; Division of Ophthalmic Pathology, Department of Ophthalmology, University Hospital Bonn, Germany.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2022 Apr; Vol. 217, pp. 108958. Date of Electronic Publication: 2022 Jan 24.
DOI: 10.1016/j.exer.2022.108958
Abstrakt: The purpose of this study was to investigate Müller cells during the fetal development of the human eye. Müller cells in eyes of 39 human fetuses (11-38 weeks of gestation, WOG) and 6 infants (5 died of abusive head trauma, AHT, aged 1-9 months) were immunohistochemically stained and investigated for spatial and temporal immunoreaction of nestin, CD44, collagen IX and GFAP, which are stem cell markers or markers of intermediate filaments, respectively, in one of the hitherto largest cohorts of fetal eyes. Müller cells could be detected immunohistochemically as early as 12 WOG by immunohistochemical staining with nestin. Nestin was more strongly expressed in Müller cells of the peripheral retina and a centroperipheral gradient of immunoreaction over time was observed. CD44 was predominantly expressed in fetal eyes of the late second and early third trimester between (23 and 27 WOG) and significantly stronger in the infant eyes. Collagen IX labeling in the central retina was significantly stronger than in more peripheral sectors and increased with fetal age. GFAP staining in Müller cells was seen in the eye of a fetus of 38 WOG who died postnatally and in the infant eyes with and without history of AHT. Additionally, GFAP staining was present in the astrocytes of fetal and infant eyes. All examined markers were expressed by Müller cells at different developmental stages highlighting the plasticity of Müller cells during the development of the human eye. GFAP should be cautiously used as a marker for AHT as it was also expressed in fetal astrocytes and Müller cells in eyes without history of AHT.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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