The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells.

Autor: Melani RD; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Gerbasi VR; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Anderson LC; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA., Sikora JW; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Toby TK; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Hutton JE; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Butcher DS; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA., Negrão F; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Seckler HS; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Srzentić K; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Fornelli L; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Camarillo JM; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., LeDuc RD; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Cesnik AJ; Department of Genetics, Stanford University, Stanford, CA, USA.; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden., Lundberg E; Department of Genetics, Stanford University, Stanford, CA, USA.; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden., Greer JB; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Fellers RT; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Robey MT; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., DeHart CJ; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Forte E; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.; Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Hendrickson CL; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA., Abbatiello SE; Thermo Fisher Scientific, Cambridge, MA, USA., Thomas PM; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA., Kokaji AI; STEMCELL Technologies Inc., Vancouver, BC, Canada., Levitsky J; Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA., Kelleher NL; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.; Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Jazyk: angličtina
Zdroj: Science (New York, N.Y.) [Science] 2022 Jan 28; Vol. 375 (6579), pp. 411-418. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1126/science.aaz5284
Abstrakt: Human biology is tightly linked to proteins, yet most measurements do not precisely determine alternatively spliced sequences or posttranslational modifications. Here, we present the primary structures of ~30,000 unique proteoforms, nearly 10 times more than in previous studies, expressed from 1690 human genes across 21 cell types and plasma from human blood and bone marrow. The results, compiled in the Blood Proteoform Atlas (BPA), indicate that proteoforms better describe protein-level biology and are more specific indicators of differentiation than their corresponding proteins, which are more broadly expressed across cell types. We demonstrate the potential for clinical application, by interrogating the BPA in the context of liver transplantation and identifying cell and proteoform signatures that distinguish normal graft function from acute rejection and other causes of graft dysfunction.
Databáze: MEDLINE
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