| Autor: |
Yang D, Ye F, Teijaro CN, Hwang D, Annaval T, Adhikari A, Li G, Yan X, Gui C, Rader C, Shen B |
| Jazyk: |
angličtina |
| Zdroj: |
Organic letters [Org Lett] 2022 Feb 11; Vol. 24 (5), pp. 1219-1223. Date of Electronic Publication: 2022 Jan 27. |
| DOI: |
10.1021/acs.orglett.2c00009 |
| Abstrakt: |
Comparative analyses of four anthraquinone-fused enediyne biosynthetic gene clusters (BGCs) identified YpmL as a cytochrome P450 enzyme unique to the yangpumicin (YPM) BGC. In vitro characterization of YpmL established it as a hydroxylase, catalyzing C-6 hydroxylation in YPM A biosynthesis. In vivo application of YpmL enabled engineered production of four new tiancimycin analogues ( 14 - 17 ). Evaluation of their cytotoxicity against selected human cancer cell lines shed new insights into the enediyne structure-activity relationship. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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