Effect of acute swimming exercise at different intensities but equal total load over metabolic and molecular responses in swimming rats.

Autor: Forte LDM; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas - UNICAMP, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil. lucas.dmf@hotmail.com., de Almeida Rodrigues N; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas - UNICAMP, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., Cordeiro AV; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas - UNICAMP, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., de Fante T; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., de Paula Simino LA; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., de Souza Torsoni A; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., Torsoni MA; Laboratory of Metabolic Disorders, School of Applied Sciences, University of Campinas, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., Gobatto CA; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas - UNICAMP, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil., Barros Manchado-Gobatto F; Laboratory of Applied Sport Physiology, School of Applied Sciences, University of Campinas - UNICAMP, St. 1300 Pedro Zaccaria, Limeira, São Paulo, 13484-350, Brazil.
Jazyk: angličtina
Zdroj: Journal of muscle research and cell motility [J Muscle Res Cell Motil] 2022 Mar; Vol. 43 (1), pp. 35-44. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1007/s10974-022-09614-4
Abstrakt: Acute metabolic and molecular response to exercise may vary according to exercise's intensity and duration. However, there is a lack regarding specific tissue alterations after acute exercise with aerobic or anaerobic predominance. The present study investigated the effects of acute exercise performed at different intensities, but with equal total load on molecular and physiological responses in swimming rats. Sixty male rats were divided into a control group and five groups performing an acute bout of swimming exercise at different intensities (80, 90, 100, 110 and 120% of anaerobic threshold [AnT]). The exercise duration of each group was balanced so all groups performed at the same total load. Gene expression (HIF-1α, PGC-1α, MCT1 and MCT4 mRNA), blood biomarkers and tissue glycogen depletion were analyzed after the exercise session. ANOVA One-Way was used to indicate statistical mean differences considering 5% significance level. Blood lactate concentration was the only biomarker sensitive to acute exercise, with a significant increase in rats exercised above AnT intensities (p < 0.000). Glycogen stores of gluteus muscle were significantly reduced in all exercised animals in comparison to control group (p = 0.02). Hepatic tissue presented significant reduction in glycogen in animals exercised above AnT (p = 0.000, as well as reduced HIF-1α mRNA and increased MCT1 mRNA, especially at the highest intensity (p = 0.002). Physiological parameters did not alter amongst groups for most tissues. Our results indicate the hepatic tissue alterations (glycogen stores and gene expressions) in response to different exercise intensities of exercise, even with the total load matched.
(© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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