The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development.

Autor: Holtappels D; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium., Fortuna KJ; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium., Moons L; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium., Broeckaert N; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium., Bäcker LE; Laboratory of Food Microbiology, KU Leuven, Leuven, Belgium., Venneman S; Proefstation voor de groenteteelt, Sint-Katelijne-Waver, Belgium., Rombouts S; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium.; Flanders Research Institute for Agriculture, Fisheries and Food, Ghent, Belgium., Lippens L; Provinciaal Proefcentrum voor de Groenteteelt Oost-Vlaanderen, Kruishoutem, Belgium., Baeyen S; Flanders Research Institute for Agriculture, Fisheries and Food, Ghent, Belgium., Pollet S; Inagro, Beitem, Belgium., Noben JP; Biomedical Research Institute and Transnational University Limburg, Hasselt University, Hasselt, Belgium., Oechslin F; Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland., Vallino M; Institute for Sustainable Plant Protection, National Research Counsil of Italy, Turin, Italy., Aertsen A; Laboratory of Food Microbiology, KU Leuven, Leuven, Belgium., Maes M; Independent Researcher, Ghent, Belgium., Van Vaerenbergh J; Flanders Research Institute for Agriculture, Fisheries and Food, Ghent, Belgium., Lavigne R; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium., Wagemans J; Laboratory of Gene Technology, KU Leuven, Leuven, Belgium.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2022 Jun; Vol. 15 (6), pp. 1762-1782. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1111/1751-7915.14004
Abstrakt: Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics-based control measures for this bacterium are unsustainable and inefficient. After establishing a representative collection of Xcc strains, we isolated and characterized bacteriophages from two clades of phages to assess their potential in phage-based biocontrol. The most promising phages, FoX2 and FoX6, specifically recognize (lipo) polysaccharides, associated with the wxc gene cluster, on the surface of the bacterial cell wall. Next, we determined and optimized the applicability of FoX2 and FoX6 in an array of complementary bioassays, ranging from seed decontamination to irrigation- and spray-based applications. Here, an irrigation-based application showed promising results. In a final proof-of-concept, a CaCl 2 -formulated phage cocktail was shown to control the outbreak of Xcc in the open field. This comprehensive approach illustrates the potential of phage biocontrol of black rot disease in Brassica and serves as a reference for the broader implementation of phage biocontrol in integrated pest management strategies.
(© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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