High soluble HLA-DQB2 levels in posttransplant serum are associated with kidney graft dysfunction.

Autor: Mine KL; Instituto de Imunogenética, Associação Fundo de Incentivo à Pesquisa, São Paulo, Brazil., de Marco R; Instituto de Imunogenética, Associação Fundo de Incentivo à Pesquisa, São Paulo, Brazil., Raimundo TRF; Instituto de Imunogenética, Associação Fundo de Incentivo à Pesquisa, São Paulo, Brazil., Ernesto JV; Instituto de Imunogenética, Associação Fundo de Incentivo à Pesquisa, São Paulo, Brazil., Medina-Pestana JO; Nephrology Division, Hospital do Rim, Universidade Federal de São Paulo, São Paulo, Brazil., Tedesco-Silva H; Nephrology Division, Hospital do Rim, Universidade Federal de São Paulo, São Paulo, Brazil., Gerbase-DeLima M; Instituto de Imunogenética, Associação Fundo de Incentivo à Pesquisa, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: International journal of immunogenetics [Int J Immunogenet] 2022 Apr; Vol. 49 (2), pp. 63-69. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1111/iji.12569
Abstrakt: HLA-DQB2 is a gene of limited polymorphism, with unknown function that presents at least two transcript variants: v1, which encodes the full-length beta-chain, and v2, which lacks exon 4 and could give rise to a soluble protein. We previously showed a strong correlation between high v2 expression in preimplantation biopsies (PIB) of kidneys from young (18- to 49-year olds) but not from old, deceased donors and 1-year posttransplant low (estimated glomerular filtration rate < 45 ml/min/1.73 m 2 ) graft function (GF). In this study, we aimed to investigate the impact of posttransplant soluble HLA-DQB2 (sDQB2) serum levels, v1 expression in PIB, and recipient HLA-DQB2 rs7453920 A/G polymorphism on GF. sDQB2 was evaluated by enzyme-linked immunosorbent assay in sera from 114 recipients, collected at least 1 year (median 2.1 years) after transplantation. Higher sDQB2 levels were observed in recipients of kidneys from young, but not from old, donors that had a ≥30% decline in GF within 1 year after blood collection for sDQB2 determination. Among the 15 recipients of kidneys from young donors with sDQB2 ≥ 1.52 ng/ml, 40% presented a ≥30% decline in GF, whereas this occurred in none of the 43 recipients with lower sDQB2 levels (p = 0.007; OR: 36.5). Expression of HLA-DQB2 variant 1, measured by reverse transcription-polymerase chain reaction (RT-PCR) in 92 PIB from young or old donors, did not significantly differ between transplants with high or low 4-year GF. HLA-DQB2 rs7453920 single nucleotide polymorphism (SNP) frequencies did not significantly differ between recipients with low or high 4-year GF. We conclude that HLA-DQB2 variant 1 expression in PIB and recipient rs7453920 SNP polymorphism are not associated with graft outcome. On the other hand, the association, in transplants of kidneys from young donors, between high posttransplant serum sDQB2 levels and decline in GF is a very interesting finding that deserves a validation study in a larger cohort.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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