Diversity of bacterial pathogens and their antimicrobial resistance profile among commensal rodents in Qatar.

Autor: Islam MM; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar. mmmohammed@mme.gov.qa.; School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu Natal, Durban, 4000, South Africa. mmmohammed@mme.gov.qa., Farag E; Ministry of Public Health, Doha, Qatar., Hassan MM; Faculty of Veterinary Medicine, Chottogram Veterinary and Animal Sciences University, Khulshi, Chattogram, 4225, Bangladesh., Enan KA; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar.; Department of Virology, Central Laboratory, The Ministry of Higher Education and Scientific Research, 7099, Khartum, Sudan., Mohammad Sabeel KV; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar., Alhaddad MM; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar., Smatti MK; Biomedical Research Center, Qatar University, Doha, Qatar., Al-Marri AM; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar., Al-Zeyara AA; Department of Animal Resources, Ministry of Municipality and Environment, Doha, Qatar., Al-Romaihi H; Ministry of Public Health, Doha, Qatar., Yassine HM; Biomedical Research Center, Qatar University, Doha, Qatar., Sultan AA; Department of Microbiology and Immunology, Weill Cornell Medicine, Cornell University, Doha, Qatar., Bansal D; Ministry of Public Health, Doha, Qatar., Mkhize-Kwitshana Z; School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu Natal, Durban, 4000, South Africa.; Division of Research Capacity Development, South African Medical Research Council, Tygerberg, Cape Town, 7505, South Africa.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2022 Jun; Vol. 46 (2), pp. 487-498. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1007/s11259-021-09876-2
Abstrakt: Rodents are sources of many zoonotic pathogens that are of public health concern. This study investigated bacterial pathogens and assessed their antimicrobial resistance (AMR) patterns in commensal rodents in Qatar. A total of 148 rodents were captured between August 2019 and February 2020, and blood, ectoparasites, and visceral samples were collected. Gram-negative bacteria were isolated from the intestines, and blood plasma samples were used to detect antibodies against Brucella spp., Chlamydophila abortus, and Coxiella burnetii. PCR assays were performed to detect C. burnetii, Leptospira spp., Rickettsia spp., and Yersinia pestis in rodent tissues and ectoparasite samples. Antimicrobial resistance by the isolated intestinal bacteria was performed using an automated VITEK analyzer. A total of 13 bacterial species were isolated from the intestine samples, namely Acinetobacter baumannii, Aeromonas salmonicida, Citrobacter freundii, Citrobacter koseri, Enterobacter aerogenes, Enterobacter cloacae, Escherichia coli, Hafnia alvei, Klebsiella pneumoniae, Providencia stuartii, Proteus mirabilis, Pseudomonas aeruginosa, and Salmonella enterica. The majority of them were E. coli (54.63%), followed by P. mirabilis (17.59%) and K. pneumoniae (8.33%). Most of the pathogens were isolated from rodents obtained from livestock farms (50.46%), followed by agricultural farms (26.61%) and other sources (22.94%). No antibodies (0/148) were detected against Brucella spp., C. abortus, or C. burnetii. In addition, 31.58% (6/19) of the flea pools and one (1/1) mite pool was positive for Rickettsia spp., and no sample was positive for C. burnetii, Leptospira spp., and Y. pestis by PCR. A total of 43 (38%) bacterial isolates were identified as multidrug resistant (MDR), whereas A. salmonicida (n = 1) did not show resistance to any tested antimicrobials. Over 50% of bacterial MDR isolates were resistant to ampicillin, cefalotin, doxycycline, nitrofurantoin, and tetracycline. The presence of MDR pathogens was not correlated with rodent species or the location of rodent trapping. Seven (11.86%) E. coli and 2 (22.2%) K. pneumoniae were extended-spectrum beta-lactamases (ESBL) producers. These findings suggest that rodents can be a source of opportunistic bacteria for human and animal transmission in Qatar. Further studies are needed for the molecular characterization of the identified bacteria in this study.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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