Immobilization of Baeyer-Villiger monooxygenase from acetone grown Fusarium sp.

Autor: Takagi M; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., T Sriwong K; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., Masuda A; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., Kawaguchi N; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., Fukui S; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., Le Viet LH; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan., Kato DI; Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima, 890-0065, Japan., Kitayama T; Faculty of Agriculture, Kindai University, 3327-204, Nakamachi, Nara, Japan., Fujii M; School of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi, Japan., Koesoema AA; Research Institute for Interdisciplinary Science (RIIS), Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama-shi, 700-8530, Japan., Matsuda T; Department of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan. tmatsuda@bio.titech.ac.jp.
Jazyk: angličtina
Zdroj: Biotechnology letters [Biotechnol Lett] 2022 Mar; Vol. 44 (3), pp. 461-471. Date of Electronic Publication: 2022 Jan 27.
DOI: 10.1007/s10529-022-03224-3
Abstrakt: Objective: A novel biocatalyst for Baeyer-Villiger oxidations is necessary for pharmaceutical and chemical industries, so this study aims to find a Baeyer-Villiger monooxygenase (BVMO) and to improve its stability by immobilization.
Results: Acetone, the simplest ketone, was selected as the only carbon source for the screening of microorganisms with a BVMO. A eukaryote, Fusarium sp. NBRC 109816, with a BVMO (FBVMO), was isolated from a soil sample. FBVMO was overexpressed in E. coli and successfully immobilized by the organic-inorganic nanocrystal formation method. The immobilization improved the thermostability of FBVMO. Substrate specificity investigation revealed that both free and immobilized FBVMO were found to show catalytic activities not only for Baeyer-Villiger oxidation of ketones to esters but also for oxidation of sulfides to sulfoxides. Furthermore, a preparative scale reaction using immobilized FBVMO was successfully conducted.
Conclusions: FBVMO was discovered from an environmental sample, overexpressed in E. coli, and immobilized by the organic-inorganic nanocrystal formation method. The immobilization successfully improved its thermostability.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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