A Joint Technology Combining the Advantages of Capillary Microsampling with Mass Spectrometry Applied to the Trans-Resveratrol Pharmacokinetic Study in Mice.

Autor: Xu Y; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Zhang SX; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Guo J; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Chen LJ; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Liou YL; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Rao T; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Peng JB; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Guo Y; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Huang WH; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Tan ZR; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Ou-Yang DS; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Zhou HH; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Zhang W; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China., Chen Y; Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, Hunan, China.; Institute of Clinical Pharmacology, Central South University, Changsha, Hunan, China.; Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha, Hunan, China.; National Clinical Research Center for Geriatric Disorders, Changsha, Hunan, China.
Jazyk: angličtina
Zdroj: Journal of analytical methods in chemistry [J Anal Methods Chem] 2022 Jan 17; Vol. 2022, pp. 5952436. Date of Electronic Publication: 2022 Jan 17 (Print Publication: 2022).
DOI: 10.1155/2022/5952436
Abstrakt: Mice are the most frequently used animals in pharmacokinetic studies; however, collecting series of blood samples from mice is difficult because of their small sizes and tiny vessels. In addition, due to the small sample size, it is problematic to perform high required quantification. Thus, present work aims to find an effective strategy for overcoming these challenges using trans-resveratrol as a tool drug. Based on the idea of a joint technology, the capillary microsampling (CMS) was chosen for blood sample collection from mice after delivery of trans-resveratrol (150 mg/kg) by gavage, and a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of trans-resveratrol and its main metabolites. All the mouse blood samples were exactly collected by CMS without obvious deviation. This provided credible samples for subsequent quantitative analysis. The HPLC-MS/MS method was found to be sensitive, accurate, and repeatable, and the pharmacokinetic parameters for all analytes were comparable with those reported in previous studies. However, the present joint technology offers the advantages of less animal damage, easy for sample preparation, and improved reliability. It has overcome some of the major limitations revealed in previous pharmacokinetic studies in mice and therefore provides a more effective option for future studies.
Competing Interests: The authors declare that they have no conflicts of interest or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Ying Xu et al.)
Databáze: MEDLINE
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