scDART-seq reveals distinct m 6 A signatures and mRNA methylation heterogeneity in single cells.
| Autor: | Tegowski M; Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710, USA., Flamand MN; Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710, USA., Meyer KD; Department of Biochemistry, Duke University School of Medicine, Durham, NC 27710, USA; Department of Neurobiology, Duke University School of Medicine, Durham, NC 27710, USA. Electronic address: kate.meyer@duke.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Molecular cell [Mol Cell] 2022 Feb 17; Vol. 82 (4), pp. 868-878.e10. Date of Electronic Publication: 2022 Jan 25. |
| DOI: | 10.1016/j.molcel.2021.12.038 |
| Abstrakt: | N 6 -methyladenosine (m 6 A) is an abundant RNA modification that plays critical roles in RNA regulation and cellular function. Global m 6 A profiling has revealed important aspects of m 6 A distribution and function, but to date such studies have been restricted to large populations of cells. Here, we develop a method to identify m 6 A sites transcriptome-wide in single cells. We uncover surprising heterogeneity in the presence and abundance of m 6 A sites across individual cells and identify differentially methylated mRNAs across the cell cycle. Additionally, we show that cellular subpopulations can be distinguished based on their RNA methylation signatures, independent from gene expression. These studies reveal fundamental features of m 6 A that have been missed by m 6 A profiling of bulk cells and suggest the presence of cell-intrinsic mechanisms for m 6 A deposition. Competing Interests: Declaration of interests K.D.M. has filed a patent application for the DART-seq technology through Duke University. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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