| Autor: |
Fonsêca JM; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil., Martins MD; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Department of Pathology, Porto Alegre, RS, Brazil., Vargas PA; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil., Silva WG; Instituto do Câncer do Estado de São Paulo - ICESP, Dental Oncology Service, São Paulo, SP Brazil., Normando AGC; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil., Palmier NR; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil., Ribeiro ACP; Instituto do Câncer do Estado de São Paulo - ICESP, Dental Oncology Service, São Paulo, SP Brazil., Brandão TB; Instituto do Câncer do Estado de São Paulo - ICESP, Dental Oncology Service, São Paulo, SP Brazil., Lopes MA; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil., Goes MF; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Department of Restorative Dentistry, Piracicaba, SP, Brazil., Santos-Silva AR; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Oral Diagnosis Department, Semiology and Oral Pathology Areas, Piracicaba, SP, Brazil. |
| Abstrakt: |
This study tested the hypothesis that head and neck radiotherapy (HNRT) impacts the immunoexpression of type I collagen, bone sialoprotein (BSP) and bone morphogenetic protein 4 (BMP4), thereby leading to micromorphological changes in the dentin-pulp complex (DPC), and promoting the onset and progression of radiation caries (RC). Twenty-two demineralized sections of carious teeth (a group of 11 irradiated teeth and a control group of 11 non-irradiated teeth) extracted from 19 head and neck cancer patients were analyzed by conventional optical microscopy and immunohistochemistry to investigate the micromorphology (cellular layer hierarchy, blood vessels, odontoblasts, fibroblasts, extracellular matrix, calcification, necrosis, reactionary dentin formation, and chronic inflammation), and the patterns of staining/immunolocalization of type I collagen, BSP and BMP4 in the dental pulp of irradiated and control samples. No significant differences attributable to the direct impact of radiotherapy were detected in DPC micromorphology between the groups. In addition, the patterns of immunohistochemical staining and immunolocalization of the proteins studied did not differ between the irradiated and the control samples for type I collagen, BSP or BMP4. This study rejected the hypothesis that HNRT directly damages dentition by changing the organic components and the microstructure of the DPC, ultimately leading to RC. |
