Evaluation of biofilm formation on acrylic resin surfaces coated with silicon dioxide: an in situ study.

Autor:	Silva RVDR; Universidade Estadual de Maringá - UEM, Department of Dentistry, Maringá, PR, Brazil., Jarros IC; Universidade Estadual de Maringá - UEM, Division of Medical Mycology, Department of Clinical Analysis, Maringá, PR, Brazil., Del Bel Cury AA; Universidade Estadual de Campinas - Unicamp, Piracicaba Dental School, Department of Prosthodontics and Periodontology, Piracicaba, SP, Brazil., Sidhu SK; Queen Mary University, Barts & The London School of Medicine and Dentistry, Centre for Oral Bioengineering, London, UK., Silva S; University of Minho, Centre of Biological Engineering, Laboratório de Investigação Rosário Oliveira, Braga, Portugal., Negri MFN; Universidade Estadual de Maringá - UEM, Division of Medical Mycology, Department of Clinical Analysis, Maringá, PR, Brazil., Pascotto RC; Universidade Estadual de Maringá - UEM, Department of Dentistry, Maringá, PR, Brazil.
Jazyk:	angličtina
Zdroj:	Brazilian oral research [Braz Oral Res] 2022 Jan 14; Vol. 36, pp. e007. Date of Electronic Publication: 2022 Jan 14 (Print Publication: 2022).
DOI:	10.1590/1807-3107bor-2022.vol36.0007
Abstrakt:	Biofilm on acrylic resin dental prostheses may cause gingival inflammation. This study evaluated the influence of a silicon dioxide coating layer applied onto acrylic resin on the adhesion of microorganisms. Blocks (5 x 5 x 3 mm) of acrylic resin were evaluated for surface roughness and divided into two groups: control (CG) and coated with silicon dioxide (LG group). The specimens were evaluated by scanning electron microscopy (n = 1) and by contact angle analysis (n = 3). For the in situ study, 20 volunteers wore acrylic palatal devices containing three samples from each group (n = 60) for 2 days. The biofilm formed was quantified by metabolic activity and total biomass using the crystal violet assay. The results were subjected to Bartlett's normality test and Gamma model with random effect for the response variable (α = 5%). The mean contact angle of the coated group was significantly lower than that of the uncoated group (p < 0.05). The metabolic activity of microorganisms in the biofilm on the blocks treated with coating was significantly lower than that of control blocks (p = 0.02). Regarding the amount of extracellular matrix produced by the microorganisms, there was no difference between the CG and LG group (p = 0.05). The application of a silicon dioxide coating on acrylic resin reduced the activity of the polymicrobial biofilm formed in situ. This coating may be advantageous for patients with conventional complete dentures or implants made of acrylic resin and who have motor difficulties that prevent them from cleaning their prostheses properly.
Databáze:	MEDLINE
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