End-to-end system for rapid and sensitive early-detection of SARS-CoV-2 for resource-poor and field-test environments using a $51 lab-in-a-backpack.

Autor: Lin EE; School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom., Razzaque UA; School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom., Burrows SA; School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom., Smoukov SK; School of Engineering and Materials Science, Queen Mary University of London, London, United Kingdom.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 Jan 26; Vol. 17 (1), pp. e0259886. Date of Electronic Publication: 2022 Jan 26 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0259886
Abstrakt: COVID-19 has exposed stark inequalities between resource-rich and resource-poor countries. International UN- and WHO-led efforts, such as COVAX, have provided SARS-CoV-2 vaccines but half of African countries have less than 2% vaccinated in their population, and only 15 have reached 10% by October 2021, further disadvantaging local economic recovery. Key for this implementation and preventing further mutation and spread is the frequency of voluntary [asymptomatic] testing. It is limited by expensive PCR and LAMP tests, uncomfortable probes deep in the throat or nose, and the availability of hardware to administer in remote locations. There is an urgent need for an inexpensive "end-to-end" system to deliver sensitive and reliable, non-invasive tests in resource-poor and field-test conditions. We introduce a non-invasive saliva-based LAMP colorimetric test kit and a $51 lab-in-a-backpack system that detects as few as 4 viral RNA copies per μL. It consists of eight chemicals, a thermometer, a thermos bottle, two micropipettes and a 1000-4000 rcf electronically operated centrifuge made from recycled computer hard drives (CentriDrive). The centrifuge includes a 3D-printed rotor and a 12 V rechargeable Li-ion battery, and its 12 V standard also allows wiring directly to automobile batteries, to enable field-use of this and other tests in low infrastructure settings. The test takes 90 minutes to process 6 samples and has reagent costs of $3.5 per sample. The non-invasive nature of saliva testing would allow higher penetration of testing and wider adoption of the test across cultures and settings (including refugee camps and disaster zones). The attached graphical procedure would make the test suitable for self-testing at home, performing it in the field, or in mobile testing centers by minimally trained staff.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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