Insights into the Impact of Gold Nanoclusters Au 10 SG 10 on Human Microglia.

Autor:	Maysinger D; Department of Pharmacology & Therapeutics, McGill University, H3G 1Y6 Montréal, Canada.; Interdisciplinary Center for Advanced Science and Technology (ICAST) at University of Split, Meštrovićevo šetalište 45, 21000 Split, Croatia., Sanader Maršić Ž; Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Republic of Croatia.; Interdisciplinary Center for Advanced Science and Technology (ICAST) at University of Split, Meštrovićevo šetalište 45, 21000 Split, Croatia., Gran ER; Department of Pharmacology & Therapeutics, McGill University, H3G 1Y6 Montréal, Canada., Shobo A; Department of Pharmacology & Therapeutics, McGill University, H3G 1Y6 Montréal, Canada., Macairan JR; Department of Chemical Engineering, McGill University, H3A 0C5 Montréal, Canada., Zhang I; Department of Pharmacology & Therapeutics, McGill University, H3G 1Y6 Montréal, Canada., Perić Bakulić M; Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM), Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia.; Interdisciplinary Center for Advanced Science and Technology (ICAST) at University of Split, Meštrovićevo šetalište 45, 21000 Split, Croatia., Antoine R; Institut Lumière Matière UMR 5306, Université Claude Bernard Lyon 1, CNRS, Univ Lyon, F-69100 Villeurbanne, France., Multhaup G; Department of Pharmacology & Therapeutics, McGill University, H3G 1Y6 Montréal, Canada., Bonačić-Kouteckỳ V; Center of Excellence for Science and Technology-Integration of Mediterranean Region (STIM), Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia.; Interdisciplinary Center for Advanced Science and Technology (ICAST) at University of Split, Meštrovićevo šetalište 45, 21000 Split, Croatia.; Chemistry Department, Humboldt University of Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany.
Jazyk:	angličtina
Zdroj:	ACS chemical neuroscience [ACS Chem Neurosci] 2022 Feb 16; Vol. 13 (4), pp. 464-476. Date of Electronic Publication: 2022 Jan 26.
DOI:	10.1021/acschemneuro.1c00621
Abstrakt:	The purpose of the current study is to uncover the impact of small liganded gold nanoclusters with 10 gold atoms and 10 glutathione ligands (Au 10 SG 10 ) on several biomarkers in human microglia. We established the links connecting the atomically precise structure of Au 10 SG 10 with their properties and changes in several biomolecules under oxidative stress. Au 10 SG 10 caused the loss of mitochondrial metabolic activity, increased lipid peroxidation and translocation of an alarmin molecule, high mobility group box 1 (HMGB1), from the nucleus to the cytosol. Molecular modeling provided an insight into the location of amino acid interaction sites with Au 10 SG 10 and the nature of bonds participating in these interactions. We show that Au 10 SG 10 can bind directly to the defined sites of reduced, oxidized, and acetylated HMGB1. Further studies with similar complementary approaches merging live-cell analyses, determination of biomarkers, and cell functions could lead to optimized gold nanoclusters best suited for diagnostic and bioimaging purposes in neuroscience.
Databáze:	MEDLINE
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