Electrochemical tracing of hypoxia glycolysis by carbon nanotube sensors, a new hallmark for intraoperative detection of suspicious margins to breast neoplasia.

Autor: Miripour ZS; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Abbasvandi F; ATMP Department Breast Cancer Research Center, Motamed Cancer Institute, ACECR Tehran Iran., Aghaee P; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., NajafiKhoshnoo S; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Faramarzpour M; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Mohaghegh P; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Hoseinpour P; SEPAS Pathology Laboratory Tehran Iran., Namdar N; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Amiri MH; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Ghafari H; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Zareie S; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Shojaeian F; Cancer Research Center Shahid Beheshti University of Medical Sciences Tehran Iran., Sanati H; Integrative Oncology Department Breast Cancer Research Center, Motamed Cancer Institute, ACECR Tehran Iran., Mapar M; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Sadeghian N; ATMP Department Breast Cancer Research Center, Motamed Cancer Institute, ACECR Tehran Iran., Akbari ME; Cancer Research Center Shahid Beheshti University of Medical Sciences Tehran Iran., Khayamian MA; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran., Abdolahad M; Nano Bio Electronic Devices Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Nano Electronic Center of Excellence, Thin Film and Nanoelectronics Lab, School of Electrical and Computer Engineering, College of Engineering University of Tehran Tehran Iran.; Cancer Institute, Imam Khomeini Hospital Tehran University of Medical Sciences Tehran Iran.; UT&TUMS Cancer Electrotechnique Research Center YAS Hospital, Tehran University of Medical Sciences Tehran Iran.
Jazyk: angličtina
Zdroj: Bioengineering & translational medicine [Bioeng Transl Med] 2021 Jun 14; Vol. 7 (1), pp. e10236. Date of Electronic Publication: 2021 Jun 14 (Print Publication: 2022).
DOI: 10.1002/btm2.10236
Abstrakt: For most people, the first step in treatment is to take out the tumor (surgery), so precise and fast diagnosis of any sign of high-risk and neoplastic cells, especially in surgical cavity margins, is significant. The frozen pathology method is the conventional standard of intraoperative diagnosis, but the low number of slides prepared from non-fixed tissues prevents us from achieving a perfect diagnosis. Although many improvements in intraoperative margin detection were achieved, still real-time detection of neoplastic lesions is crucial to improving diagnostic quality. Functionalized carbon nanotubes grown on the electrode needles lively and selectively determine the H 2 O 2 released from cancer/atypical cells through reverse Warburg effect and hypoxia assisted glycolysis pathways in a quantitative electrochemical manner. The study was carried out on cell lines, 57 in vivo mice models with breast cancer, and 258 fresh in vitro samples of breast cancer tumors. A real-time electrotechnical system, named cancer diagnostic probe (CDP) (US Patent Pub. No.: US 2018/02991 A1, US 2021/0007638 A1, and US 2021/0022650 A1 [publications], and US 10,786,188 B1 [granted]), has been developed to find pre-neoplastic/neoplastic cells in vivo in a quantitative electrochemical manner by tracing hypoxia glycolysis byproducts. Matched pathological evaluations with response peaks of CDP were found based on the presence of neoplasia (from atypia to invasive carcinoma) in live breast tissues. The ability of CDP to find neoplastic lesions in mice models in vivo and fresh breast tumors in vitro was verified with sensitivity and specificity of 95% and 97%, respectively. The system may help a surgeon assistant system for usage in the operating room after passing many trials and standard examinations in the future.
Competing Interests: Four USA patents (one granted; US Patent US10,786,188 B1, and three publications; US Patent App. US2018/0299401 A1, US2021/0007638 A1, and US2021/0022650 A1) have been published based on this work. M.A. is a member of the scientific advisory board of Arya Nano biosensor Manufacturer Co., a company that is commercializing CDP technology. The remaining authors declare that they have no competing interests.
(© 2021 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.)
Databáze: MEDLINE
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