Comparative assessment of the results of detection of СagА-positive strains of Helicobacter pylori by molecular-genetic and immunochromatographic methods in different biological materials.
| Autor: | Smirnova DN; Vyatka State University., Bogacheva NV; Vyatka State University.; Kirov State Medical University., Petrov SB; Kirov State Medical University., Tuneva NA; Kirov State Medical University. |
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| Jazyk: | angličtina |
| Zdroj: | Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2022 Jan 21; Vol. 67 (1), pp. 48-52. |
| DOI: | 10.51620/0869-2084-2022-67-1-48-52 |
| Abstrakt: | A comparative analysis of the detection of CagA-positive strains of H. pylori by immunochromatographic and molecular genetic methods was carried out. We used H. pylori strains isolated from individuals with diseases of the gastrointestinal tract. The immunochromatographic method was implemented using a developed experimental model of an immunochromatographic test system for detecting the H. pylori CagA protein in various biological materials. Determination of the pathogenicity gene cagA of H. pylori was carried out using the «Helikopol SA» test system («Litekh», Russia). The assessment of the comparability of the results of detecting CagA-positive strains of H. pylori was carried out using statistical methods: Monte-Carlo, calculation of the chi-square test (χ2) and Kendall's τ-b and Somer's d coefficients. Statistical analysis was performed using the software packages «Microsoft Office Excel», «Statistica 10.0», «WinBUGS 1.4.0.» The study showed the absence of a statistically significant difference and the presence of a direct strong correlation between the results of detecting CagA-positive strains by molecular genetic and immunochromatographic methods, which indicates that these methods provide similar results in identifying highly pathogenic strains of H. pylori. Competing Interests: The authors declare no conflict of interest. |
| Databáze: | MEDLINE |
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