Introduction of BD Vacutainer ® Barricor™ tubes in clinical biobanking and application of amino acid and cytokine quality indicators to Barricor plasma.

Autor:	Knutti N; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany., Neugebauer S; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany., Scherr F; Institute of Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Jena, Germany., Mathay C; Integrated BioBank of Luxembourg (IBBL), Dudelange, Luxembourg., Marchese M; Integrated BioBank of Luxembourg (IBBL), Dudelange, Luxembourg., Henry E; Integrated BioBank of Luxembourg (IBBL), Dudelange, Luxembourg., Palm J; Institute of Medical Statistics, Computer and Data Science, Jena University Hospital, Jena, Germany., Betsou F; Integrated BioBank of Luxembourg (IBBL), Dudelange, Luxembourg.; Laboratoire National de Santé (LNS), Dudelange, Luxembourg., Kiehntopf M; Institute of Clinical Chemistry and Laboratory Diagnostics and Integrated Biobank Jena (IBBJ), Jena University Hospital, Jena, Germany.
Jazyk:	angličtina
Zdroj:	Clinical chemistry and laboratory medicine [Clin Chem Lab Med] 2022 Jan 21; Vol. 60 (5), pp. 689-700. Date of Electronic Publication: 2022 Jan 21 (Print Publication: 2022).
DOI:	10.1515/cclm-2021-0899
Abstrakt:	Objectives: The use of BD Vacutainer ® Barricor™ tubes (BAR) can reduce turnaround time (TAT) and improve separation of plasma from cellular components using a specific mechanical separator. Concentrations of amino acids (AAs) and cytokines, known to be labile during pre-analytical time delays, were compared in heparin (BAR, BD Heparin standard tube [PST]), EDTA and serum gel tubes (SER) to validate previously identified quality indicators (QIs) in BAR. Methods: Samples of healthy individuals (n=10) were collected in heparin, EDTA and SER tubes and exposed to varying pre- and post-centrifugation delays at room temperature (RT). Cytokines (interleukin [IL]-8, IL-16 and sCD40L) were analyzed by enzyme-linked immunosorbent assay (ELISA) and AAs were characterized by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results: All QIs, AAs/AA ratio and cytokines increased during prolonged blood storage in heparin plasma (PST, BAR) and SER tubes. Comparison of 53 h/1 h pre-centrifugation delay resulted in an increase in taurine (Tau) and glutamic acid (Glu) concentrations by more than three times, soluble CD40L increased by 13.6, 9.2 and 4.3 fold in PST, BAR-CTRL and BAR-FAST, and IL-8 increased even more by 112.8 (PST), 266.1 (BAR-CTRL), 268.1 (BAR-FAST) and 70.0 (SER) fold, respectively. Overall, compared to prolonged blood storage, effects of post-centrifugation delays were less pronounced in all tested materials. Conclusions: BAR tubes are compatible with the use of several established QIs and can therefore be used in clinical biobanking to reduce pre-analytical TAT without compromising QIs and thus pre-analytical sample quality analysis. (© 2022 Walter de Gruyter GmbH, Berlin/Boston.)
Databáze:	MEDLINE
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