Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles.

Autor:	Seufert P; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany., Staiger S; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany., Arand K; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany., Bueno A; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany., Burghardt M; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany., Riederer M; Chair of Botany II - Ecophysiology and Vegetation Ecology, Julius von Sachs Institute of Biological Sciences, University of Würzburg, Würzburg, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Jan 05; Vol. 12, pp. 766602. Date of Electronic Publication: 2022 Jan 05 (Print Publication: 2021).
DOI:	10.3389/fpls.2021.766602
Abstrakt:	Waxes are critical in limiting non-stomatal water loss in higher terrestrial plants by making up the limiting barrier for water diffusion across cuticles. Using a differential extraction protocol, we investigated the influence of various wax fractions on the cuticular transpiration barrier. Triterpenoids (TRPs) and very long-chain aliphatics (VLCAs) were selectively extracted from isolated adaxial leaf cuticles using methanol (MeOH) followed by chloroform (TCM). The water permeabilities of the native and the solvent-treated cuticles were measured gravimetrically. Seven plant species ( Camellia sinensis , Ficus elastica , Hedera helix , Ilex aquifolium , Nerium oleander , Vinca minor, and Zamioculcas zamiifolia ) with highly varying wax compositions ranging from nearly pure VLCA- to TRP-dominated waxes were selected. After TRP removal with MeOH, water permeability did not or only slightly increase. The subsequent VLCA extraction with TCM led to increases in cuticular water permeabilities by up to two orders of magnitude. These effects were consistent across all species investigated, providing direct evidence that the cuticular transpiration barrier is mainly composed of VLCA. In contrast, TRPs play no or only a minor role in controlling water loss. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Seufert, Staiger, Arand, Bueno, Burghardt and Riederer.)
Databáze:	MEDLINE
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