Caffeine Protects Against Retinal Inflammation.

Autor: Conti F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Lazzara F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Romano GL; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Platania CBM; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy., Drago F; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy., Bucolo C; Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.; Center for Research in Ocular Pharmacology-CERFO, University of Catania, Catania, Italy.
Jazyk: angličtina
Zdroj: Frontiers in pharmacology [Front Pharmacol] 2022 Jan 06; Vol. 12, pp. 824885. Date of Electronic Publication: 2022 Jan 06 (Print Publication: 2021).
DOI: 10.3389/fphar.2021.824885
Abstrakt: Caffeine, one of the most consumed central nervous system (CNS) stimulants, is an antagonist of A 1 and A 2A adenosine receptors. In this study, we investigated the potential protective effects of this methylxanthine in the retinal tissue. We tested caffeine by using in vitro and in vivo paradigms of retinal inflammation. Human retinal pigment epithelial cells (ARPE-19) were exposed to lipopolysaccharide (LPS) with or without caffeine. This latter was able to reduce the inflammatory response in ARPE-19 cells exposed to LPS, attenuating the release of IL-1β, IL-6, and TNF-α and the nuclear translocation of p-NFκB. Additionally, caffeine treatment restored the integrity of the ARPE-19 monolayer assessed by transepithelial electrical resistance (TEER) and the sodium fluorescein permeability test. Finally, the ischemia reperfusion (I/R) injury model was used in C57BL/6J mice to induce retinal inflammation and investigate the effects of caffeine treatment. Mouse eyes were treated topically with caffeine, and a pattern electroretinogram (PERG) was used to assess the retinal ganglion cell (RGC) function; furthermore, we evaluated the levels of IL-6 and BDNF in the retina. Retinal BDNF dropped significantly ( p < 0.05) in the I/R group compared to the control group (normal mice); on the contrary, caffeine treatment maintained physiological levels of BDNF in the retina of I/R eyes. Caffeine was also able to reduce IL-6 mRNA levels in the retina of I/R eyes. In conclusion, these findings suggest that caffeine is a good candidate to counteract inflammation in retinal diseases.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Conti, Lazzara, Romano, Platania, Drago and Bucolo.)
Databáze: MEDLINE
Externí odkaz: