Multi-drug resistance, integron and transposon-mediated gene transfer in heterotrophic bacteria from Penaeus vannamei and its culture environment.

Autor: Nadella RK; MFB Division, ICAR-Central Institute of Fisheries Technology, Willingdon Island, Matsyapuri P.O., Cochin, 682029, Kerala, India., Panda SK; QAM Division, ICAR-Central Institute of Fisheries Technology, Willingdon Island, Matsyapuri P.O, Cochin, 682029, Kerala, India., Badireddy MR; Visakhapatnam Research Centre, ICARCentral Institute of Fisheries Technology, Visakhapatnam, 530003, Andhra Pradesh, India., Kurcheti PP; Aquatic Environment and Health Management Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India., Raman RP; Aquatic Environment and Health Management Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, 400061, Maharashtra, India., Mothadaka MP; MFB Division, ICAR-Central Institute of Fisheries Technology, Willingdon Island, Matsyapuri P.O., Cochin, 682029, Kerala, India. prasadmm@hotmail.com.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 May; Vol. 29 (25), pp. 37527-37542. Date of Electronic Publication: 2022 Jan 23.
DOI: 10.1007/s11356-021-18163-1
Abstrakt: Multi-drug resistance (MDR) in bacteria is regarded as an emerging pollutant in different food production avenues including aquaculture. One hundred and sixty out of 2304 bacterial isolates from shrimp farm samples (n = 192) of Andhra Pradesh, India, were MDR. Based on biochemical identification and 16S rRNA sequencing, they were grouped into 35 bacterial species with the predominance of Vibrio parahaemolyticus (12.5%). The MDR isolates showed highest resistance toward oxytetracycline (89%) with more than 0.2 MAR (multiple antibiotic resistance), demonstrates a high-risk source. The most prevalent antibiotic-resistance gene (ARG) and mobile genetic element (MGE) detected were tetA (47.5%) and int1 (46.2%), respectively. In conjugation experiments, overall transfer frequency was found to be in the range of 1.1 × 10 -9 to 1.8 × 10 -3 with the transconjugants harbouring ARGs and MGEs. This study exposed the wide distribution of MDR bacteria in shrimp and its environment, which can further aggravate the already raised concerns of antibiotic residues in the absence of proper mitigation measures.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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