Relation of shear stress and K L a on bikaverin production by Fusarium oxysporum CCT7620 in a bioreactor.

Autor: Dos Santos MC; Department of Food Science, Faculty of Food Engineering, State University of Campinas, Rua Monteiro Lobato, 80, Campinas, SP, 13083-862, Brazil., Cerri MO; Department of Bioprocess and Biotechnology, UNESP Universidade Estadual Paulista, Araraquara, SP, Brazil., Bicas JL; Department of Food Science, Faculty of Food Engineering, State University of Campinas, Rua Monteiro Lobato, 80, Campinas, SP, 13083-862, Brazil. jlbicas@gmail.com.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2022 Apr; Vol. 45 (4), pp. 733-740. Date of Electronic Publication: 2022 Jan 23.
DOI: 10.1007/s00449-022-02693-0
Abstrakt: This study involved evaluating the effects of rotational impeller speed agitation (N) and specific air flow rate (Ф air ) on bikaverin production and on the growth of Fusarium oxysporum employing 11 bench-scale bioreactor assays. The results showed that the maximum bikaverin production (close to 300 mg L -1 ) was achieved after 48 h of fermentation in rice medium (20 g L -1 milled rice in water) at 28 °C with a volumetric oxygen transfer coefficient (K L a) and shear stress values of approximately 20 h -1 and 17 N m -2 , respectively. We reached this combination of parameters using an N of 340 rpm and Ф air of 0.935 vvm. These K L a and shear stress values can be used as references when upscaling this process. Thus, this study was important to demonstrate how the main parameters in bioreactors affect bikaverin production and it presented important indications for upscaling this bioprocess.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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