Oxidative stress and inflammatory markers in patients with COVID-19: Potential role of RAGE, HMGB1, GFAP and COX-2 in disease severity.

Autor: Passos FRS; Laboratory of Neurosciences and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil., Heimfarth L; Laboratory of Neurosciences and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil., Monteiro BS; Laboratory of Neurosciences and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Corrêa CB; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil; Department of Morphology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Moura TR; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil; Department of Morphology, Federal University of Sergipe, São Cristóvão, SE, Brazil., Araújo AAS; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil; Department of Pharmacy, Federal University of Sergipe, São Cristóvão, SE, Brazil., Martins-Filho PR; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil; Investigative Pathology Laboratory, Federal University of Sergipe, Aracaju, SE, Brazil., Quintans-Júnior LJ; Laboratory of Neurosciences and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: lucindo@academico.ufs.br., Quintans JSS; Laboratory of Neurosciences and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, SE, Brazil. Electronic address: jullyanas@academico.ufs.br.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2022 Mar; Vol. 104, pp. 108502. Date of Electronic Publication: 2022 Jan 05.
DOI: 10.1016/j.intimp.2021.108502
Abstrakt: Background: SARS-CoV-2 infection can lead to the abnormal induction of cytokines and a dysregulated hyperinflammatory state that is implicated in disease severity and risk of death. There are several molecules present in blood associated with immune cellular response, inflammation, and oxidative stress that could be used as severity markers in respiratory viral infections such as COVID-19. However, there is a lack of clinical studies evaluating the role of oxidative stress-related molecules including glial fibrillary acidic protein (GFAP), the receptor for advanced glycation end products (RAGE), high mobility group box-1 protein (HMGB1) and cyclo-oxygenase-2 (COX-2) in COVID-19 pathogenesis.
Aim: To evaluate the role of oxidative stress-related molecules in COVID-19.
Method: An observational study with 93 Brazilian participants from September 2020 to April 2021, comprising 23 patients with COVID-19 admitted to intensive care unit (ICU), 19 outpatients with COVID-19 with mild to moderate symptoms, 17 individuals reporting a COVID-19 history, and 34 healthy controls. Blood samples were taken from all participants and western blot assay was used to determine the RAGE, HMGB1, GFAP, and COX-2 immunocontent.
Results: We found that GFAP levels were higher in patients with severe or critical COVID-19 compared to outpatients (p = 0.030) and controls (p < 0.001). A significant increase in immunocontents of RAGE (p < 0.001) and HMGB1 (p < 0.001) were also found among patients admitted to the ICU compared to healthy controls, as well as an overexpression of the inducible COX-2 (p < 0.001). In addition, we found a moderate to strong correlation between RAGE, GFAP and HMGB1 proteins.
Conclusion: SARS-CoV-2 infection induces the upregulation of GFAP, RAGE, HMGB1, and COX-2 in patients with the most severe forms of COVID-19.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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