Negeviruses isolated from mosquitoes in the Brazilian Amazon.

Autor: da Silva Ribeiro AC; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Martins LC; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., da Silva SP; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., de Almeida Medeiros DB; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Miranda KKP; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Nunes Neto JP; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., de Oliveira Monteiro HA; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., do Nascimento BLS; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Junior JWR; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Cruz ACR; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., da Costa Vasconcelos PF; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil., Carvalho VL; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil. valeriacarvalho@iec.gov.br., Rodrigues SG; Department of Arbovirology and Hemorrhagic Fevers and Postgraduate Program in Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n, Levilândia, Ananindeua, Pará, 67030-000, Brazil.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2022 Jan 21; Vol. 19 (1), pp. 17. Date of Electronic Publication: 2022 Jan 21.
DOI: 10.1186/s12985-022-01743-z
Abstrakt: Background: There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.
Methods: Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.
Results: Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.
Conclusions: We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.
(© 2022. The Author(s).)
Databáze: MEDLINE
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