| Autor: |
Chaves BA; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Godoy RSM; Instituto de Pesquisas René Rachou, Fiocruz, Belo Horizonte 30190-002, Brazil., Campolina TB; Instituto de Pesquisas René Rachou, Fiocruz, Belo Horizonte 30190-002, Brazil., Júnior ABV; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Paz ADC; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Vaz EBDC; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Silva BM; Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Brazil., Nascimento RM; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Guerra MDGVB; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Lacerda MVG; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil.; Instituto de Pesquisas René Rachou, Fiocruz, Belo Horizonte 30190-002, Brazil., Monteiro WM; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil., Secundino NFC; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil.; Instituto de Pesquisas René Rachou, Fiocruz, Belo Horizonte 30190-002, Brazil., Pimenta PFP; Fundação de Medicina Tropical Heitor Vieira Dourado, Manaus 69040-000, Brazil.; Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69850-000, Brazil.; Instituto de Pesquisas René Rachou, Fiocruz, Belo Horizonte 30190-002, Brazil. |
| Abstrakt: |
The successful spread and maintenance of the dengue virus (DENV) in mosquito vectors depends on their viral infection susceptibility, and parameters related to vector competence are the most valuable for measuring the risk of viral transmission by mosquitoes. These parameters may vary according to the viral serotype in circulation and in accordance with the geographic origin of the mosquito population that is being assessed. In this study, we investigated the effect of DENV serotypes (1-4) with regards to the infection susceptibility of five Brazilian Ae. aegypti populations from Manaus, the capital of the state of Amazonas, Brazil. Mosquitoes were challenged by oral infection with the DENV serotypes and then tested for the presence of the arbovirus using quantitative PCR at 14 days post-infection, which is the time point that corresponds to the extrinsic incubation period of Ae. aegypti when reared at 28 °C. Thus, we were able to determine the infection patterns for DENV-1, -2, -3 and -4 in the mosquito populations. The mosquitoes had both interpopulation and inter-serotype variation in their viral susceptibilities. All DENV serotypes showed a similar tendency to accumulate in the body in a greater amount than in the head/salivary gland (head/SG), which does not occur with other flaviviruses. For DENV-1, DENV-3, and DENV-4, the body viral load varied among populations, but the head/SG viral loads were similar. Differently for DENV-2, both body and head/SG viral loads varied among populations. As the lack of phenotypic homogeneity represents one of the most important reasons for the long-term fight against dengue incidence, we expect that this study will help us to understand the dynamics of the infection patterns that are triggered by the distinct serotypes of DENV in mosquitoes. |
