DNA Metabarcoding from Microbial Communities Recovered from Stream and Its Potential for Bioremediation Processes.

Autor: Ottoni JR; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Dos Santos Grignet R; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Barros MGA; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Bernal SPF; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Panatta AAS; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Lacerda-Júnior GV; Brazilian Agricultural Research Corporation, Embrapa Meio Ambiente, Jaguariúna, Brazil., Centurion VB; Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), Campinas State University (UNICAMP), Paulínia, São Paulo, 13148-218, Brazil., Delforno TP; Innovation Institute for Biotechnology (SENAI-Biotech), Rua Anhaia, 1321 - Bom Retiro - São Paulo, São Paul, CEP: 01130-000, Brazil., da Costa Silva Goncalves C; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil., Passarini MRZ; Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil. michel.passarini@unila.edu.br.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2022 Jan 20; Vol. 79 (2), pp. 70. Date of Electronic Publication: 2022 Jan 20.
DOI: 10.1007/s00284-021-02752-x
Abstrakt: Urban waste (UW) has caused a series of problems regarding its management. UW comprises domestic, hospital and industrial residues, which makes the destination of this waste a matter of concern, as it may contain a variety of highly toxic environmental polluters. Deactivated dumps can represent sources of contamination of the environment that surround these deposits, harming rivers and inhabiting organisms. Knowledge of the microbial profile of water bodies that can be affected by these toxic residues is essential for the development of alternatives and improvements in treatments applied in rivers and streams. In this sense, this work aimed to analyze the microbial community present in sediments of the Arroio Dourado stream in the municipality of Foz do Iguaçu, a stream located near a deactivated open-air dump. 16S rDNA metabarcoding suggested the dominance of acidogenic bacteria belonging to Acidobacteriota phylum, followed by less abundant phyla Actinobacteriota, Myxococcota, Chloroflexi and a small community of sulfate reducers (Desulfobacteriota). However, more than 50% of amplicon sequence variants (ASVs) were not taxonomically classified. In addition, an expressive abundance was attributed to the genus Anaeromyxobacter, a metabolically versatile group, which can thrive in the presence of polluting compounds present in the deactivated landfill. Thus, a possible stream treatment process can be developed. In addition, culture media can be developed for the recovery of taxonomic groups identified involved in the biodegradation of organic compounds. The results presented expand the knowledge of bacterial diversity in sediment samples recovered from the Arroio Dourado stream.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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