Asymmetric patterning drives the folding of a tripodal DNA nanotweezer.

Autor:	Saliba D; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., Trinh T; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., Lachance-Brais C; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., Prinzen AL; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., Rizzuto FJ; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., de Rochambeau D; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca., Sleiman HF; Department of Chemistry, McGill University 801 rue Sherbrooke West Montreal QC H3A 0B8 Canada hanadi.sleiman@mcgill.ca.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2021 Nov 16; Vol. 13 (1), pp. 74-80. Date of Electronic Publication: 2021 Nov 16 (Print Publication: 2021).
DOI:	10.1039/d1sc04793k
Abstrakt:	DNA tweezers have emerged as powerful devices for a wide range of biochemical and sensing applications; however, most DNA tweezers consist of single units activated by DNA recognition, limiting their range of motion and ability to respond to complex stimuli. Herein, we present an extended, tripodal DNA nanotweezer with a small molecule junction. Simultaneous, asymmetric elongation of our molecular core is achieved using polymerase chain reaction (PCR) to produce length- and sequence-specific DNA arms with repeating DNA regions. When rigidified, our DNA tweezer can be addressed with streptavidin-binding ligands. Full control over the number, separation, and location of these ligands enables site-specific streptavidin recognition; all three arms of the DNA nanotweezer wrap around multiple streptavidin units simultaneously. Our approach combines the simplicity of DNA tile arrays with the size regime normally provided by DNA origami, offering an integrated platform for the use of branched DNA scaffolds as structural building blocks, protein sensors, and dynamic, stimuli-responsive materials. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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