| Autor: |
Hasan N; College of Pharmacy, Pusan National University, Busan 46241, Korea.; Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia., Lee J; College of Pharmacy, Pusan National University, Busan 46241, Korea., Ahn HJ; School of Mechanical and Aerospace Engineering, Gyeongsang National University, Jinju 52828, Korea., Hwang WR; School of Mechanical and Aerospace Engineering, Gyeongsang National University, Jinju 52828, Korea., Bahar MA; Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia., Habibie H; Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia., Amir MN; Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia., Lallo S; Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia., Son HJ; College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 627706, Korea., Yoo JW; College of Pharmacy, Pusan National University, Busan 46241, Korea. |
| Abstrakt: |
Polymicrobial wound infections are a major cause of infectious disease-related morbidity and mortality worldwide. In this study, we prepared a nitric oxide (NO)-releasing oxidized bacterial cellulose/chitosan (BC TO /CHI) crosslinked hydrogel to effectively treat polymicrobial wound infections. Linear polyethyleneimine diazeniumdiolate (PEI/NO) was used as the NO donor. The aldehyde group of BC TO and the amine of CHI were used as crosslinked hydrogel-based materials; their high NO loading capacity and antibacterial activity on the treatment of polymicrobial-infected wounds were investigated. The blank and NO-loaded crosslinked hydrogels, namely BC TO -CHI and BC TO -CHI-PEI/NO, were characterized according to their morphologies, chemical properties, and drug loading. BC TO -CHI-PEI/NO exhibited sustained drug release over four days. The high NO loading of BC TO -CHI-PEI/NO enhanced the bactericidal efficacy against multiple bacteria compared with BC TO -CHI. Furthermore, compared with blank hydrogels, BC TO -CHI-PEI/NO has a favorable rheological property due to the addition of a polymer-based NO donor. Moreover, BC TO -CHI-PEI/NO significantly accelerated wound healing and re-epithelialization in a mouse model of polymicrobial-infected wounds. We also found that both crosslinked hydrogels were nontoxic to healthy mammalian fibroblast cells. Therefore, our data suggest that the BC TO -CHI-PEI/NO developed in this study improves the efficacy of NO in the treatment of polymicrobial wound infections. |
