| Autor: |
Rossi MM; Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy., Matturro B; Water Research Institute (IRSA-CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy., Amanat N; Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy., Rossetti S; Water Research Institute (IRSA-CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy., Petrangeli Papini M; Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. |
| Jazyk: |
angličtina |
| Zdroj: |
Microorganisms [Microorganisms] 2022 Jan 04; Vol. 10 (1). Date of Electronic Publication: 2022 Jan 04. |
| DOI: |
10.3390/microorganisms10010101 |
| Abstrakt: |
Towards chlorinated solvents, the effectiveness of the remediation strategy can be improved by combining a biological approach (e.g., anaerobic reductive dechlorination) with chemical/physical treatments (e.g., adsorption). A coupled adsorption and biodegradation (CAB) process for trichloroethylene (TCE) removal is proposed in a biofilm-biochar reactor (BBR) to assess whether biochar from pine wood (PWB) can support a dechlorinating biofilm by combining the TCE (100 µM) adsorption. The BBR operated for eight months in parallel with a biofilm reactor (BR)-no PWB (biological process alone), and with an abiotic biochar reactor (ABR)-no dechlorinating biofilm (only an adsorption mechanism). Two flow rates were investigated. Compared to the BR, which resulted in a TCE removal of 86.9 ± 11.9% and 78.73 ± 19.79%, the BBR demonstrated that PWB effectively adsorbs TCE and slows down the release of its intermediates. The elimination of TCE was quantitative, with 99.61 ± 0.79% and 99.87 ± 0.51% TCE removal. Interestingly, the biomarker of the reductive dechlorination process, Dehalococcoides mccartyi , was found in the BRR (9.2 × 10 5 16S rRNA gene copies/g), together with the specific genes tceA , bvcA , and vcrA (8.16 × 10 6 , 1.28 × 10 5 , and 8.01 × 10 3 gene copies/g, respectively). This study suggests the feasibility of biochar to support the reductive dechlorination of D. mccartyi , opening new frontiers for field-scale applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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