| Autor: |
Sitkov N; Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Zimina T; Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Kolobov A; Institute of Highly Pure Biopreparations, 197110 Saint Petersburg, Russia., Sevostyanov E; Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Trushlyakova V; Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Luchinin V; Department of Micro- and Nanoelectronics, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Krasichkov A; Radio Engineering Systems Department, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia., Markelov O; Centre for Digital Telecommunication Technologies, Saint Petersburg Electrotechnical University 'LETI', 5 Professor Popov Street, 197376 Saint Petersburg, Russia., Galagudza M; Almazov National Research Centre, 197341 Saint Petersburg, Russia., Kaplun D; Department of Automation and Control Processes, Saint Petersburg Electrotechnical University 'LETI', 197376 Saint Petersburg, Russia. |
| Abstrakt: |
A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system's sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine. |
