Oleanolic Acid Alleviates Cerebral Ischemia/Reperfusion Injury via Regulation of the GSK-3β/HO-1 Signaling Pathway.

Autor: Lin K; School of Public Health, Guangzhou Medical University, Guangzhou 511436, China., Zhang Z; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Zhang Z; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Zhu P; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Jiang X; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Wang Y; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Deng Q; Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 511436, China., Lam Yung KK; Department of Biology, Faculty of Science, Hong Kong Baptist University (HKBU), Kowloon Tong, Hong Kong 999077, China.; Golden Meditech Center for Neuro Regeneration Sciences, HKBU, Kowloon Tong, Hong Kong 999077, China., Zhang S; JNU-HKUST Joint Laboratory for Neuroscience and Innovative Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2021 Dec 21; Vol. 15 (1). Date of Electronic Publication: 2021 Dec 21.
DOI: 10.3390/ph15010001
Abstrakt: Oleanolic acid (OA), a bioactive ingredient of Panax ginseng , exhibits neuroprotective pharmacological effects. However, the protective role of OA in cerebral ischemia and involved mechanisms remain unclear. This study attempted to explore the therapeutic effects of OA both in vitro and in vivo. OA attenuated cytotoxicity and overproduction of intracellular reactive oxygen species (ROS) by regulation of glycogen synthase kinase-3β (GSK-3β)/heme oxygenase-1 (HO-1) signal in oxygen-glucose deprivation/reoxygenation (OGD/R)-exposed SH-SY5Y cells. Additionally, OA administration significantly reduced the area of cerebral infarction and the neurological scores in the rat models of cerebral ischemia with middle cerebral artery occlusion (MCAO). The OA administration group showed a higher percentage of Nissl + and NeuN + cells, along with lower TUNEL + ratios in the infarct area of MCAO rats. Moreover, OA administration reduced ROS production while it suppressed the GSK-3β activation and upregulated the HO-1 expression in infarcted tissue. Our results illustrated that OA significantly counteracted cerebral ischemia-mediated injury through antioxidant effects induced by the regulation of the GSK-3β/HO-1 signaling pathway, implicating OA as a promising neuroprotective drug for the therapy of ischemic stroke.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje