Standard Methods for Dissection of Varroa destructor Females.

Autor: Piou V; Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France., Vilarem C; Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France.; M2i Biocontrol-Entreprise SAS, 46214 Parnac, France., Rein C; Apicultural State Institute, University of Hohenheim, Erna-Hruschka-Weg 6, 70599 Stuttgart, Germany., Sprau L; Department of Livestock Population Genomics, University of Hohenheim, Garbenstr. 17, 70599 Stuttgart, Germany., Vétillard A; Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France.
Jazyk: angličtina
Zdroj: Insects [Insects] 2021 Dec 29; Vol. 13 (1). Date of Electronic Publication: 2021 Dec 29.
DOI: 10.3390/insects13010037
Abstrakt: Varroa destructor (Anderson and Trueman) is known as a major pest of Apis mellifera L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of V. destructor mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females V. destructor dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in V. destructor research.
Databáze: MEDLINE
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