| Autor: |
Volova TG; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia.; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia., Prudnikova SV; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia., Kiselev EG; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia.; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia., Nemtsev IV; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia.; L.V. Kirensky Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/38 Akademgorodok, 660036 Krasnoyarsk, Russia.; Federal Research Center 'Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences', 50 Akademgorodok, 660036 Krasnoyarsk, Russia., Vasiliev AD; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia.; L.V. Kirensky Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/38 Akademgorodok, 660036 Krasnoyarsk, Russia., Kuzmin AP; School of Petroleum and Gas Engineering, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia., Shishatskaya EI; School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny Pr., 660041 Krasnoyarsk, Russia.; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 50/50 Akademgorodok, 660036 Krasnoyarsk, Russia. |
| Abstrakt: |
The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m 2 and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923. |
