| Autor: |
Pérez-Monter C; Departamento de Gastroenterología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico., Álvarez-Arce A; Departamento de Neuropatología Molecular, División de Neurociencias, Instituto de Fisiología Celular, UNAM, Mexico City 04510, Mexico., Nuño-Lambarri N; Unidad de Investigación Traslacional, Fundación Clínica Médica Sur, Mexico City 14050, Mexico., Escalona-Nández I; Departamento de Gastroenterología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico., Juárez-Hernández E; Unidad de Investigación Traslacional, Fundación Clínica Médica Sur, Mexico City 14050, Mexico., Chávez-Tapia NC; Unidad de Investigación Traslacional, Fundación Clínica Médica Sur, Mexico City 14050, Mexico., Uribe M; Unidad de Investigación Traslacional, Fundación Clínica Médica Sur, Mexico City 14050, Mexico., Barbero-Becerra VJ; Unidad de Investigación Traslacional, Fundación Clínica Médica Sur, Mexico City 14050, Mexico. |
| Abstrakt: |
Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances. The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content. Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model. Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin. Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing. Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group. We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia muciniphila increased by 5-fold with inulin supplementation. The family Prevotellaceae was also significantly increased in the fat-diet group. Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis. |
