| Autor: |
Zhuravleva IY; E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya Street, 630055 Novosibirsk, Russia., Dokuchaeva AA; E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya Street, 630055 Novosibirsk, Russia., Karpova EV; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia., Timchenko TP; E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya Street, 630055 Novosibirsk, Russia., Titov AT; V. Sobolev Institute of Geology and Mineralogy SB RAS, 3 Academician Koptyug Avenue, 630090 Novosibirsk, Russia., Shatskaya SS; Institute of Solid State Chemistry and Mechanochemistry SB RAS, 18 Kutateladze Street, 630128 Novosibirsk, Russia., Polienko YF; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia. |
| Abstrakt: |
Calcification is the major factor limiting the clinical use of bioprostheses. It may be prevented by the immobilization of bisphosphonic compounds (BPs) on the biomaterial. In this study, we assessed the accumulation and structure of calcium phosphate deposits in collagen-rich bovine pericardium (Pe) and elastin-rich porcine aortic wall (Ao) and bovine jugular vein wall (Ve) cross-linked with glutaraldehyde (GA) or diepoxy compound (DE). These tissues were then modified with pamidronic (PAM) acid or 2-(2'-carboxyethylamino)ethylidene-1,1-bisphosphonic (CEABA) acid. Tissue transformations were studied using Fourier-transform infrared spectroscopy. After subcutaneous implantation of the biomaterials in 220 rats, calcification dynamics were examined using atomic absorption spectrophotometry, light microscopy after von Kossa staining, and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy The calcium content in all GA-cross-linked tissues and DE-cross-linked Ao increased to 100-160 mg/g on day 60 after implantation. BPs prevented the accumulation of phosphates on the surface of all materials and most effectively inhibited calcification in GA-cross-linked Ao and DE-cross-linked Pe. PAM containing -OH in the R1 group was more effective than CEABA containing -H in R1. The calcification-inhibitory effect of BPs may be realized through their ability to block nucleation and prevent the growth of hydroxyapatite crystals. |
