| Autor: |
Araujo AC; Graduate Program in Animal Sciences, State University of Southwestern Bahia, Itapetinga 45700-000, Brazil.; Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA., Carneiro PLS; Department of Biology, State University of Southwest Bahia, Jequié 45205-490, Brazil., Alvarenga AB; Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA., Oliveira HR; Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA.; Centre for Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON N1G2W1, Canada., Miller SP; American Angus Association, Angus Genetics Inc., 3201 Frederick Ave, St. Joseph, MO 64506, USA., Retallick K; American Angus Association, Angus Genetics Inc., 3201 Frederick Ave, St. Joseph, MO 64506, USA., Brito LF; Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA. |
| Abstrakt: |
Behavior is a complex trait and, therefore, understanding its genetic architecture is paramount for the development of effective breeding strategies. The objective of this study was to perform traditional and weighted single-step genome-wide association studies (ssGWAS and WssGWAS, respectively) for yearling temperament (YT) in North American Angus cattle using haplotypes. Approximately 266 K YT records and 70 K animals genotyped using a 50 K single nucleotide polymorphisms (SNP) panel were used. Linkage disequilibrium thresholds (LD) of 0.15, 0.50, and 0.80 were used to create the haploblocks, and the inclusion of non-LD-clustered SNPs (NCSNP) with the haplotypes in the genomic models was also evaluated. WssGWAS did not perform better than ssGWAS. Cattle YT was found to be a highly polygenic trait, with genes and quantitative trait loci (QTL) broadly distributed across the whole genome. Association studies using LD-based haplotypes should include NCSNPs and different LD thresholds to increase the likelihood of finding the relevant genomic regions affecting the trait of interest. The main candidate genes identified, i.e., ATXN10 , ADAM10 , VAX2 , ATP6V1B1 , CRISPLD1 , CAPRIN1 , FA2H , SPEF2 , PLXNA1 , and CACNA2D3 , are involved in important biological processes and metabolic pathways related to behavioral traits, social interactions, and aggressiveness in cattle. Future studies should further investigate the role of these candidate genes. |
